blastocyst formation

Conclusions: 1.Sequential culture system can acquire higher blastocyst formation rate and implantation rate, so it may replace coculture system.

结论：1.序贯培养较共培养有更高的囊胚形成率和植入率，并且IGF-Ⅱ测定结果表明序贯培养的胚胎具有较高的发育及植入潜能。

The blastocyst formation rate was 32.9% using G1/ G2 sequential culture media.

采用G1/G2序贯培养，囊胚形成率为32.9%；

The 2-cell mouse embryo were added to the stroma cells after cultured 8 days. The embryo growth rate and blastocyst formation rate 、 hatching 、 adhereing 、 outspreading of mouse embryo and the development of stroma cells were observed under the microscope.

内膜培养8天后，加入小鼠2-细胞期胚胎继续培养，观察胚胎的发育情况及内膜细胞生长情况。

Embryos after culture ever day , the blastocyst formation rate and hatching rate were accounted.

结果输卵管积水组的胚胎成囊率和囊胚孵出率低于无积水组,并呈剂量依赖性.

Compared with single embryo culture group, three embryo group cultivation increased quality blastocyst formation rate ( P0.05).

与单个胚胎培养组相比较，三个胚胎培养组的优质囊胚形成率增高（P0.05）。

The result showed that the mouse embryonic fibroblasts ( MEF) group achieved significantly higher blastocyst formation rate ( 38.

结果表明：胎鼠成纤维细胞（MEF）培养组的囊胚率和胚胎细胞数（38。

Gene Expression and Regulation in Blastocyst Formation

囊胚形成的基因调控

SP significantly promoted the embryo growth rate ( after 8-cell) and blastocyst formation rate 、 hatching 、 adhering 、 outspreading on the endometrial stroma cells ( p < 0.05).

精浆能促进八细胞以后的胚胎在子宫内膜细胞上的卵裂、囊胚形成、孵化、黏附和扩展性生长（p

To further clarify the role of egg mutation mechanism, and improve the process of mouse blastocyst formation of molecular control theory to lay a certain foundation.

为进一步阐明卵子突变基因的作用机理，完善小鼠胚泡形成过程中分子调控的相关理论奠定了一定的基础。

This research is, to Ms Payne's knowledge, the first systematic study by time-lapse recording of blastocyst formation.

据Payne女士称，该研究是首次利用实时监控录像对囊胚形成进行系统的研究。